Effect of Hormonal Steroids on the Uptake of Labeled Amino Acids by HeLa Cells

概要

論文の詳細を見る
The uptake of glycine-1-^<14>C and AIB-1-^<14>C by HeLa cells into the 60% alcoholsoluble fraction and the effect of steroid (10 μg/ml of medium) on it were studied. After exposure of the cells to cortisol for 48 hr during culture, glycine uptake by the cells in 3,5,10 and 60 min of incubation in lactalbumin medium was depressed to 55 to 84% of the control. Larger depressing effect of cortisol on glycine uptake in 10 min of incubation was observed when the cells were incubated in Eagle's medium. Deoxycorticosterone also decreased glycine uptake to 55.6% of the control in 10 min of incubation in Eagle's medium. Cortisol decreased AIB uptake by 33 and 41%, and deoxycorticosterone by 9% (not significant) and 21% in 10 and 30 min of incubation, respectively. 17β-Estradiol and estriol did not significantly affect glycine uptake.
公益社団法人日本薬学会の論文
1971-01-25