ラット肝細胞の浮遊培養(第2報) : 酵素誘導への応用

概要

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Rat liver cell suspension, prepared by the previously described method, ^<3)> was cultured in a PEB medium supplemented with bovine serum on a gyratory shaker for induction of enzymes. Tryptophan (4.2mM) and hydrocortisone (30μg/ml) added to the culture medium increased markedly the tryptophan pyrrolase activity of the cell suspension after 4-7 hours of culture. Puromycin blocked the maintenance and increase of this enzyme activity in spite of the presence of hydrocortisone. Treatment of liver cells with phenobarbita1 in vitro resulted in lowering of aminopyrine N-demethylase activity over a long period of time but the activity again appeared by further culture of liver cells ; after 25 hours in the male liver and around 36 hours in the female liver. In the case of male liver cells not treated with phenobarbital, this lowered activity did not recover even after 31 hours of culture. Testosterone (30μg/ml) did not accelerate this action of phenobarbital in female rat liver cells.
公益社団法人日本薬学会の論文
1970-05-25