Transaminases of Hepatic Tissue Culture Cells and the Effect of Carbon Tetrachloride on Their Leakage

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The direct effect of CCl_4 on leakage of cellular glutamic oxaloacetic and glutamic pyruvic transaminases of a line of hepatic tissue culture cell, HTC cell, into medium was quantitatively studied. Cellular levels of soluble isozymes of the both aminotransferases were considerably lowered by addition of CCl_4 to culture medium at a concentration of 10 mM followed by 48 and 96 hr of culture. Contrasting increase or less influenced decrease of enzyme activities in bathing fluid after treatment made the leaking indexes of the enzymes (cellular secretion of activities found in medium/cellular activities) twice or more as large as control depending on the concentrations of CCl_4 in medium and lengths of treatment. Simulating study on change of CCl_4 concentration in bathing fluid with time of culture showed a rapid and extensive fall of it upon the addition of CCl_4 with a later stable phase probably in equilibration with the agent in the gas phase within culture flask.
公益社団法人日本薬学会の論文
1977-05-25