Further Studies on the P-450 Difference Spectra induced by Various Compounds in Liver Microsomes from 3-Methylcholanthrene Treated Rats

概要

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Substrate induced difference spectra in microsomes from 3-methylcholanthrene treated rats were investigated using substrates which cause type I difference spectrum in microsomes from control rats. It was found that α, α'-dipyridyl showed only type II spectrum, whereas SKF 525-A and ethylmorphine produced type I spectra. Additionally, hexobarbital, aminopyrine and imipramine produced type I spectra when they were used by rather low concentrations but type II spectra were induced by high concentrations of them. α, α'-Dipyridyl caused type II spectrum when microsomes from control rats were treated with phospholipase C. The type II spectrum of α, α'-dipyridyl in 3-methylcholanthrene-induced microsomes was increased by treatment with phospholipase C. Moreover, α, α'-dipyridyl showed type II spectrum in microsomes from control rats when SKF 525-A, which has a high affinity to combine with type I binding site of P-450,was added into reference and sample cuvettes. From above data, the authors concluded that most of type I compounds could combine with both type I and type II binding sites, and discussed on the properties of type I and type II binding sites.
社団法人日本薬学会の論文
1973-01-25