ラット肝細胞の浮遊培養(第1報) : 培養法の基礎的検討

概要

論文の詳細を見る
Suspensions of rat hepatic cord cells were prepared by homogenizing rat liver previously perfused with Locke's solution (Ca^<2+>-free) containing citrate in 0.25M sucrose with a loose homogenizer, and then by filtering the homogenate through a 200 mesh gauze. Then the cells were cultured in Eagle (Ca^<2+>-free) or PEB medium (Table I) supplemented with bovine serum in 150 ml bottles, under stirring at 37°, the air phase has been replaced by a mixture of O_2 (95%) and CO_2 (5%). Hepatic cells of about 30 μ in diameter maintained their morphological appearance and population throughout 48 hr of culture when PEB medium supplemented with 20% (v/v) or more of serum was used. Oxygen-consuming activity of isolated mitochondria was evident in cells cultured up to 6 hr. The activities of succinic dehydrogenase and glucose-6-phosphatase of the cells decreased markedly in the first day of culture.
公益社団法人日本薬学会の論文
1970-05-25