Monitoring Differential Changes of the Mobility of Cell Membrane Components Following Treatment with a Tumor Promoter by Using Pyrene and a Novel Fatty Acid Fluorophore
スポンサーリンク
概要
- 論文の詳細を見る
The effects of an active tumor promoter, 12-O-tetradecanoyl phorbol-13-acetate (TPA), on cell membrane components have been examined using two lipophilic fluorophores, pyrene and 12-[6-(N-methylanilino)-2-naphthalenesulfonyloxy]-stearic acid (MANSOS), in Chang liver cells. MANSOS has a high fluorescence quantum yield in water and shows a blue shift (15nm from the value of 445nm in phosphate buffer) of the emission maximum on incorporation into the cell membrane.Pretreatment of cells with TPA for 2 h induced differential changes in the fluorescence parameters of pyrene and MANSOS incorporated into the cell membrane; it decreased the lifetime and harmonic mean of the rotational relaxation time and amplified the quanching by KI of pyrene fluorescence, whereas it increased the harmonic mean of the rotational relaxation time and slightly attenuated the quenching of MANSOS fluorescence.Taken together, the findings suggest different partition characteristics of the two fluorescent probes in the cell membrane components, and differential effects of TPA on the two species of components as judged from the opposite mobility changes of the two probes.
- 公益社団法人日本薬学会の論文
- 1986-05-25
著者
-
大屋敷 孝雄
北陸大・薬
-
大屋敷 孝雄
Department of Biochemistry, School of Pharmacy, Hokuriku University
-
毛利 哲郎
Department of Physiological Chemistry, School of Pharmacy, Hokuriku University
-
毛利 哲郎
2nd Division:the Research Laboratory For Development Of Medicine School Of Pharmacy Hokuriku Univers
-
毛利 哲郎
Department Of Biochemical Pharmacology Faculty Of Pharmaceutical Sciences University Of Chiba:(prese
-
高寺 恒雄
Department of Physiological Chemistry, Hokuriku University, School of Pharmacy
-
川中 明宏
Department of Physiological Chemistry, Hokuriku University, School of Pharmacy
-
川中 明宏
Department Of Physiological Chemistry Hokuriku University School Of Pharmacy
関連論文
- 肝ミクロソーム膜G-6-Pase活性に及ぼす過酸化脂質分解産物の影響
- Effects of Lipid Peroxidation on Surface Charge Density of the Porcine Intestinal Brush-Border Membranes
- Changes in Surface Charge Density of Lecithin Liposomes by Lipid Peroxidation. A Fluorescence Study with 8-Anilino-1-naphthalenesulfonate
- Antioxidant Effect of Vitamin K Homologues on Ascorbic Acid/Fe^-induced Lipid Peroxidation of Lecithin Liposomes
- A Conformation Change of the Porcine Intestinal Calcium Binding Protein on Binding of Ca^
- Changes in the Fluorescence Characteristics of N-(1-Pyrene) Maleimide Bound to the Intestinal Brush-Border Membranes by Neuraminidase Treatment
- Transaminases of Hepatic Tissue Culture Cells and the Effect of Carbon Tetrachloride on Their Leakage
- Dexamethasone, Prednisolone各エステルの比較研究 : リン酸および硫酸エステルの抗Carrageenin浮腫効果とステロイドの血中濃度変化
- Effect of Hormonal Steroids on the Uptake of Labeled Amino Acids by HeLa Cells
- PC12細胞におけるCaイオノフォアによる細胞障害と活性酸素
- Monitoring Differential Changes of the Mobility of Cell Membrane Components Following Treatment with a Tumor Promoter by Using Pyrene and a Novel Fatty Acid Fluorophore
- Fluorometric Analysis of the Micelle Formation Process of Surfactants in Aqueous Solution. II. Interaction of Cholate Molecules during Premicelle Formation
- Fluorometric Analysis of the Micelle Formation Process of Surfactants in Aqueous Solution. I. Utility of Pyrene in Determination of the Critical Micelle Concentration
- 蛍光測定の生体膜研究への応用
- Fluorometric Analysis of Micelle Formation of Sodium Dodecyl Sulfate
- ラット肝細胞の浮遊培養(第2報) : 酵素誘導への応用
- ラット肝細胞の浮遊培養(第1報) : 培養法の基礎的検討
- Negative Effect of Thalidomide and Relative Substances on the Growth of HeLa Cells