Participation of Lipid Peroxides in Rat Pertussis Vaccine Pleurisy. II. : Leucocytes and Related Enzymes

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The peroxidation of lipids and the activities of related enzymes, such as superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px), the migration of leucocytes in the pleural space, and the release of superoxide by leucocytes were studied in a rat model of Bordetella pertussis vaccine pleurisy.A pleural injection of B. pertussis vaccine into sensitized rats was found to be associated with a transient increase of lipid peroxidation in the exudate and the serum, but the level of thiobarbituric acid-reactive substance rapidly diminished.The diminution of lipid peroxidation was not relatd to the activity of GSH-Px but was related to the activity of SOD. The migration of leucocytes into the pleural cavity increased gradually and reached the maximum value 96h after the intrapleural challenge, but the release of superoxide by leucocytes was not correlated with the number of leucocytes. The number of polymorphonuclear cells (PMN) reached the maximum value 24h later, then gradually decreased, and mononuclear cells replaced PMN. Mononuclear cells were more numerous than PMN in the leucocyte population of the exudate in vaccine-induced pleurisy. The release of superoxide by leucocytes reached the maximum value 24h later, then decreased gradually. Therefore, the production rate of supoxide by PMN was larger than that by mononuclear cells.A principal component analysis of these parameters was carried out. It has been suggested that the inflammatory process of pertussis vaccine pleurisy can be divided into three phases. The first response (0-24h) represents the acute phase response; this phase has the characteristic of a predominantly PMN population in the exudate and there is marked superoxide generation or lipid peroxidation. The second phase of the response (24-96h) represents the delayed-type reaction; this phase has the characteristic of a predominantly mononuclear cell population in the exudate. The third phase of the response (96-144h) represents the healing process of the inflammatory reaction.
社団法人日本薬学会の論文
1988-07-25

Participation of Lipid Peroxides in Rat Pertussis Vaccine Pleurisy. II. : Leucocytes and Related Enzymes

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