Fluorometric Analysis of Biological Materials. I. A Fluorophotometric Determination of Carbohydrates Using Taurine and Borate

概要

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Taurine was found to react with carbohydrates by heating at neutral pH giving intense fluorescence. The development of fluorescence was proved to be greatly enhanced by addition of borate in the reaction mixture. This reaction was specific for the reducing sugars having C-2 hydroxyl or C-2 amino groups. The fluorescence intensity of oligosaccharides decreased with the increase in the degree of polymerization of monosaccharide units. Application of these findings provided a rapid, sensitive and reproducible assay method for carbohydrates. Standard curve for glucose was linear in the range of final concentration of 0.1 to 10.0 μg/ml of the sample and the coefficient of variation of 6.0 μg/ml of glucose was 1.4%.
社団法人日本薬学会の論文
1978-04-25