Microanalysis of Proteins and Peptides. I. Enhancement of the Fluorescence Intensity of Dansyl Amino Acids and Dansyl Proteins in Aqueous Media and Its Application to Assay of Amino Acids and Proteins
スポンサーリンク
概要
- 論文の詳細を見る
The fluorescence of dansyl amino acids and dansyl proteins in aqueous media was found to be greatly enhanced by addition of cycloheptaamylose. This finding was successfully applied for improvement of the assay procedure of amino acids and proteins. Dansyl amino acids of 50 picomoles per ml in final concentration was estimated. Interaction of cycloheptaamylose with dansyl amino acids was thermodynamically studied. The entropies and the enthalpies were found to vary with polarities of the dansyl amino acid.
- 公益社団法人日本薬学会の論文
- 1974-10-25
著者
-
辻 章夫
School of Pharmaceutical Sciences, Showa University
-
木下 俊夫
School of Pharmaceutical Sciences, Kitasato University
-
辻 章夫
School Of Pharmaceutical Sciences Showa University
-
飯沼 文夫
帝京大学薬学部薬品分析学教室
-
木下 俊夫
School Of Pharmaceutical Sciences Kitasato University
-
飯沼 文夫
School of Pharmaceutical Sciences, Showa University
関連論文
- Fluorescence Enzyme Immunoassay of 17α-Hydroxyprogesterone in Dried Blood Samples on Filter Paper and Its Application to Mass Screening for Congenital Adrenal Hyperplasia
- タウリン-ホウ酸試薬を用いる薄層および高速液体クロマトグラフィ-における糖類の蛍光分析 (糖質の化学)
- Determination of Serum Uric Acid and Glucose Using Dichlorofluorescin-Cycloheptaamylose Complex
- Fluorometric Analysis of Biological Materials. I. A Fluorophotometric Determination of Carbohydrates Using Taurine and Borate
- A Sensitive Method for the Fluorometric Analysis of Phenylthiohydantoin Amino Acids using Hypochlorite-Thiamine Reagent
- A Novel Method for the Fluorometric Assay of Proteins using Hypochlorite-Thiamine Reagent
- Enzymatic Formation of Xanthurenic Acid 8-Methyl Ether, an Endogenous Carcinogen, in Animal Tissues
- FLUOROMETRIC DETERMINATION OF UNSATURATED DISACCHARIDES PRODUCED ENZYMATICALLY FROM GLYCOSAMINOGLYCANS AND SEPARATED BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
- 高速液体クロマトグラフィーによる生体試料中の3-ヒドロキシアントラニル酸の蛍光定量(超微量分析のための前処理と予備濃縮)
- 酢酸亜鉛(II)試薬を用いる尿中キヌレン酸の蛍光分析
- Simultaneous Fluorometric and Colorimetric Detection of Carbohydrates on Silica Gel Plates using o-Aminobenzenesulfonic Acid
- Microdetermination of Hexosamines
- Microdetermination of Glucose using 3-Methyl-2-benzothiazolone Hydrazone Hydrochloride and Glucose Oxidase-Catalase Enzyme System
- A Direct Reductive Deamination of Amidines with Sodium Borohydride. Formation of Deaminated Compounds and Secondary Amines
- Fluorometric Determination of Hydrogen Peroxide with 4-Amino-1H-1,5-benzodiazepine-3-carbonitrile Hydrochloride
- 3,3'-Bipyridazine Derivatives as Spectrophotometric Reagents for Iron. II
- Fluorescence Enzyme Immunoassay for Insulin using Peroxidase-Tyramine-Hydrogen Peroxide
- Highly Sensitive Biotin-Labelled Hybridization Probe
- Fluorophotometric Assay of Proteins using Hypochlorite-Thiamine Reagent
- Ennancement of Fluorescence Intensity of Dansyl Amino Acids on Silica Gel Plate using Cycloheptaamylose
- 次亜鉛素第三ブチルを用いるアミド基、イミド基、あるいはアミノ基を有する医薬品の薄層クロマトグラム上における微量検出法
- Application of Quenching of Albumin-Induced Fluorescence of 8-Anilinonaphthalene-1-sulfonate to the Studies on Protein Bindings and Protein-Mucopolysaccharide Interaction
- Enhancement of the Fluorescence Intensity of Dansyl Protamine in the Presence of Sulfated Carbohydrates and Its Application to Assay of Mucopolysaccharides and Synthetic Sulfates of Mono-, Oligo-, and Polysaccharides
- Fluorophotometric Determination of Cycloheptaamylose and Its Application to the Studies on Solubilization of the Cycloamylose with Urea
- Microanalysis of Proteins and Peptides. II. Fluorescent Labeling of Proteins and a Plasma Membrane Using Cycloheptaamylose-Dansyl Chloride Complex
- Microanalysis of Proteins and Peptides. I. Enhancement of the Fluorescence Intensity of Dansyl Amino Acids and Dansyl Proteins in Aqueous Media and Its Application to Assay of Amino Acids and Proteins
- A Novel Method for the Determination of Chinoform
- Studies on Protein Bindings utilizing Quenching of Albumin-Induced Fluorescence of 8-Anilinonaphthalene-1-sulfonate
- Analytical Chemical Studies on Amino Sugars. IV. Determination of 2-Deoxy-2-sulfamido-D-glucose and 2-Acetamido-2-deoxy-D-glucose in Heparin
- Analytical Chemical Studies on Amino Sugars. II. Deterimation of Hexosamines using 3-Methyl-2-benzothiazolone Hydrazone Hydrochloride
- Analytical Chemical Studies on Amino Sugars. I. New Color Reaction of Hexosamines using p-Nitrobenzaldehyde and Tetraethylammonium Hydroxide
- 3-Oxoglucuronic Acid. III. Synthesis of 3-Amino-3-deoxy-D-allouronic Acid
- New Color Reaction of Hexosamines
- 3-Ketoglucuronic Acid. II. Constitution and Chemical Properties of 3-Ketoglucuronic Acid
- 3-Ketoglucuronic Acid. I. Synthesis of 3-Ketoglucuronic Acid
- Synthesis of 3-Keto-D-glucuronic Acid
- Synthesis of Methyl 1,2-O-Isopropylidene-3-oxo-5-O-acetyl-D-glucofuranuronate
- 他核NMRスペクトルのデーターベース作成 第二報 Pt-195NMR その1
- A Fluorophotometric Method for the Determination of Pyridoxal and Its Phosphate by Using Glycine and Zinc (II) Ion
- シクロヘプタアミロース-グアニジン系に溶解したフルオレセインイソチオシアネートを用いるたん白質のメンブランフィルター上でのけい光分析
- ウリカーゼ-ペルオキシダーゼ系と4-メトキシ-1-ナフトールとを用いる尿酸の定量法
- The Molecular Structure of (N-Salicylidene Ethanolaminato) Zn (II)
- CHEMILUMINESCENCE ENZYME IMMUNOASSAY OF 17α-HYDROXYPROGESTERONE USING GLUCOSE OXIDASE AND BIS (2,4,6-TRICHLOROPHENYL) OXALATE-FLUORESCENT DYE SYSTEM
- A Fluorophotometric Determination of Serum Creatinine and Creatine using a Creatinineamidohydrolase-Creatineamidinohydrolase-Sarcosine Oxidase-Peroxidase System and Diacetyldichlorofluorescin