Microanalysis of Proteins and Peptides. II. Fluorescent Labeling of Proteins and a Plasma Membrane Using Cycloheptaamylose-Dansyl Chloride Complex
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概要
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A complex between cycloheptaamylose and dansyl chloride was prepared and the product (CDC) was applied to the fluorescent labeling of proteins in aqueous media. Excellent degree of labeling was achieved without using any organic solvent. CDC was found to be moderately soluble in the presence of excess cycloheptaamylose and highly soluble in the presence of urea in aqueous solution. CDC-cycloheptaamylose system was successfully applied to the fluorescent labeling of rat liver plasma membrane. CDC in urea solution provided dansylation method for proteins in a homogeneous system. Rapid dansylation was observed in all the methods described.
- 公益社団法人日本薬学会の論文
- 1974-10-25
著者
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辻 章夫
School of Pharmaceutical Sciences, Showa University
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木下 俊夫
School of Pharmaceutical Sciences, Kitasato University
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辻 章夫
School Of Pharmaceutical Sciences Showa University
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飯沼 文夫
帝京大学薬学部薬品分析学教室
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木下 俊夫
School Of Pharmaceutical Sciences Kitasato University
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飯沼 文夫
School of Pharmaceutical Sciences, Showa University
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