A Novel Method for the Fluorometric Assay of Proteins using Hypochlorite-Thiamine Reagent
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概要
- 論文の詳細を見る
A fluorometric assay method for proteins based on the reaction of peptide groups is described. The N-H groups of peptides are chlorinated with sodium hypochlorite, and the resulting N-chloropeptides are allowed to react with thiamine, giving fluorescent thiochrome. The present method is highly sensitive and is applicable to 1-10μg of protein. Little variability of fluorescence intensity was observed among proteins.
- 社団法人日本薬学会の論文
- 1980-02-25
著者
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辻 章夫
School of Pharmaceutical Sciences, Showa University
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木下 俊夫
School of Pharmaceutical Sciences, Kitasato University
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村山 純一郎
School Of Pharmaceutical Sciences Showa University
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村山 和代
Yuki Gosei Kogyo Company Ltd.
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辻 章夫
School Of Pharmaceutical Sciences Showa University
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木下 俊夫
School Of Pharmaceutical Sciences Kitasato University
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