MICROSPECTROPHOTOMETRIC MEASUREMENT OF DNA ON ISOLATED EPIDERMAL CELLS, COMBINATED WITH ^3H-TdR AUTORADIOGRAPHY
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(1) Feulgen cytophotometry was performed selectively on sufficient number of single whole nuclei of epidermal cells which were isolated from a sheet of stripped epidermis. (2) The two-wavelength-scanning-method using 550nm and 450nm made it possible to detect and eliminate non-specific light loss. (3) The two-wavelength-scanning-method using the Olympus-MMSP-TU-S spectrophotometer enables us to identify the DNA synthetic cells with their intermediate values of absorbance. (4) In normal epidermis of a rabbit's ear, 90.2% of all basal cells exist in G_1-phase (including G_0-phase), and 2.9% of them are in G_2-phase. The remaining 6.9% are DNA synthetic cells. (5) The absence of "G_2-population of Gelfant" was confirmed in normal epidermis of a rabbit's ear. (6) The rate of DNA synthesis is once decreased in the middle of S-phase and it is accelerated again toward the end. This phenomenon may be related to the late-replicating DNA. (7) Autoradiographic study with ^3H-TdR on a sheet of stripped epidermis enabled us to obtain a bird's eye -view of distribution of proliferating basal cells.
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