Characterization and Environmental Regulation of a 24 kDa Cornus sericea Dehydrin-like Protein and its Relationship to Freeze-Tolerance (セミナー ストレスと生物の応答・適応--その分子・細胞機構と応用)

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概要

• 論文の詳細を見る

• Using the extremely freeze-tolerant woody shrub C. sericea as a model system, we investigated the association of xylem proteins to cold acclimation. We identified a predominant 24 kDa dehydrin-like protein that accumulated during cold acclimation, raised a polyclonal antibody and screened a cDNA expression library to search for related genes. In controlled environment conditions, we identified and deciphered the mode of regulation for the 24 kDa protein by manipulating abiotic factors and monitoring subsequent physiological and molecular effects. With immunolocalization techniques, we clarified the subcellular localization of 24 kDa-like proteins in an effort to provide insight into a potential biological role. To assess the proposed role of dehydrins to freeze induced desiccation (caused by extracellular ice formation), we characterized freezing behavior within 30 Cornus species into classifications of either supercooling or non-supercooling behaviors. Subsequent comparisons of total xylem protein blot analysis determined that 24 kDa-like proteins were not limited to species that display non-supercooling behavior, nor to those species that are extremely freeze-tolerant.

• 低温生物工学会の論文
• 2003-08-30

著者

• Itoh Takao

  Wood Res. Inst. Kyoto Univ.

• Fujino Takeshi

  Wood Research Institute, Kyoto University

• Fujino T

  Wood Research Institute Kyoto University

• Fujino Takeshi

  Department Of Electric And Electronic Engineering Ritsumeikan University

• Joly Robert

  Department Of Horticulture And Landscape Architecture Purdue University

• KARLSON Dale

  Department of Horticulture and Landscape Architecture, Purdue University

• SARNIGHAUSEN Eric

  Department of Horticulture and Landscape Architecture, Purdue University

• ZENG Yan

  Department of Horticulture and Landscape Architecture, Purdue University

• RHAGOTHAMA K.

  Department of Horticulture and Landscape Architecture, Purdue University

• STIRM Vicki

  Department of Horticulture and Landscape Architecture, Purdue University

• ASHWORTH Edward

  Department of Horticulture and Landscape Architecture, Purdue University

• Stirm Vicki

  Department Of Horticulture And Landscape Architecture Purdue University

• Karlson Dale

  Department Of Horticulture And Landscape Architecture Purdue University:(present Address)division Of

• Karlson Dale

  Department Of Horticulture And Landscape Architecture Purdue University

• Rhagothama K.

  Department Of Horticulture And Landscape Architecture Purdue University

• Ashworth Edward

  Department Of Horticulture And Landscape Architecture Purdue University

• Sarnighausen Eric

  Department Of Horticulture And Landscape Architecture Purdue University:(present Address)institut Fu

• Fujino Takeshi

  Wood Research Institute Kyoto University

• Zeng Yan

  Department Of Horticulture And Landscape Architecture Purdue University:(present Address)va Medical

• Zeng Yan

  Department Of Horticulture And Landscape Architecture Purdue University

• Baba Kei'ichi

  Kyoto Univ. Kyoto Jpn

• Fujino Takeshi

  Wood Res. Inst. Kyoto Univ.

• Baba Kei'ichi

  Rish & Institute Of Sustainability Science Kyoto University

• JOLY Robert

  Department of Horticulture and Landscape Architecture, Purdue University

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