Orientation of Microtubules during Regeneration of Cell Wall in Selected Giant Marine Algae

概要

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The microtubules in highly synchronized aplanospores of two giant marine algae, Boergesenia forbesii and Valonia ventricosa, were examined by immunofluorescence microscopy throughout the regeneration of the cell wall. Microtubule orientation was always random up to 20 h after wounding, although the orientation of cellulose microfibrils changed from random to parallel within that time period. When the rhizoid cells were in the stage of elongation at 7 to 10 days after wounding, highly ordered microtubules were always observed along the longitudinal cell axis except at the very tip of the cells where random ones were found. In contrast, the microfibrils in the innermost lamellae of newly synthesized cell' walls showed three different orientations, that is, transverse, longitudinal and oblique to the longitudinal cell axis. These observations suggest that microtubules may control cell shape, but not the orientation of microfibrils. The mechanism of cell wall construction in these algae is discussed in relation to the self-assembly mechanism thought to operate in the construction of helicoidal cell walls.
日本植物生理学会の論文