ガスパーミエーション・ガスクロマトグラフィーを用いる尿中アンモニアの迅速簡易定量

概要

論文の詳細を見る
Rapid determination of ammonia in urine was investigated. The apparatus was mainly composed of a gas chromatograph and a thermostat in which a permeation plate assembly was installed. The gas permeation membrane used in the assembly was Fluo ropore (Sumitomo Electric Ind., Tokyo) of 0.45*m pore size. The membrane was sandwitched in between two stainless steel plates. Passage for gas or liquid was an U-shaped ditch carved in a circle on the surface of the each steel plate. Two thin plates of silicone rubber for gas tight sealing were inserted separately between the steel plate and the membrane. An urine sample of 500 *l was injected into the stream of 1/15M phosphate buffer solution (pH 6.5) and carried in two the permeation plate assembly with the buffer solution. Ammonia in the sample was permeated from the buffer solution through the membrane into GC carrier gas (He). The permeated ammonia was determined by GC. The precision forthe measurement was good and within 6 % in terms of coefficient of variation. A detection limit was 10 Hg to amount of ammonia in the injected sample. An analytical time required for ammonia determination was within 20 min and it was 25 min shorter in comparison with that of the indophenol method. A correlation between the both methods was also good and the correlation coefficient was 0.97 (n=12).
社団法人日本分析化学会の論文
1982-03-05