^1H-核磁気共鳴スペクトルによるウシ胎児血清中フェツインの2-ピリジルアミノ化O-結合糖鎖の分析

概要

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The standard sugars (GalNAc, Galβ1-3GalNAc) in the core region of O-linked oligo-saccharides were labeled with a fluorescent reagent, 2-aminopyridine, by reductive amination. These sugars were then analyzed by ^1H-NMR spectroscopy. The chemical shifts and spectral pattern of the structural-reporter groups of the pyridylaminated sugars agreed with that of the structural-reporter groups of the corresponding O-linked sugar-alditols, except for the H-2 and the N-acetyl protons of N-acetylgalactosamine. It was proven that the structural-reporter groups of the O-linked sugar-alditols are applicable to a structural analysis of the corresponding pyridylaminated sugars. The structures of GalNAc-PA and Galβ1-3GalNAc-PA, prepared from fetuin in fetal calf serum, were analyzed using this method.
社団法人日本分析化学会の論文
1997-08-05