低pHキャピラリー電気泳動法による植物中のベタインアルデヒドの定量(「特集」キャピラリー電気泳動法の新展開)

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A simple and rapid determination method for betaine aldehyde in plants was developed by capillary electrophoresis (CE). After betaine aldehyde was converted to the stable p-nitrobenzyl oxime, the reaction mixture was applied directly to CE. A low pH of around 3 of the migration buffer, which lowers the electroosmotic flow, was found to be favorable for the migration of positively charged compounds. This resulted in a high separation not only between the oximes, but also between the reagent and products. This also gave another advantage of omitting a pretreatment procedure of the reaction mixture. Betaine aldehyde oxime was well determined by either 214 or 270 nm within 15 minutes. A good linearity was shown in concentrations ranging from 0.10 to 10.0 mM. The repeatability (n=5) of the migration time and the peak area were 1.11% and 5.26% as RSD, respectively. The method was applied to barley and common bean samples cultured with or without salt-stress, and proved to be suitable for the determination of a trace and unstable constituent, betaine aldehyde.
社団法人日本分析化学会の論文
1997-06-05