ジメチルスルホキシド還元酵素によるスルホキシドの速度論的分割のためのミセル動電クロマトグラフィー (<特集> バイオサイエンスと分析化学)

概要

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Recently, micellar electrokinetic chromatography (MEKC) has been successfully applied to the separation of electrically neutral compounds. In this study, we applied this method in order to determine simultaneously organic sulfoxide and sulfide; benzyl methyl sulfoxide (BMSO) and benzyl methyl sulfide (BMS), respectively. Dimethyl sulfoxide (DMSO) reductase from Rhodobacter sphaeroides f.sp.denitrificans deoxygenates (S)-BMSO preferably. The MEKC method gave reliable estimates of BMSO concentration without any pretreatment, therefore, the (R)-isomer could be recovered with high enantiomeric excess by stopping the reaction at the conversion rate of 50%. Using this detection method, (R)-BMSO was recovered with good chemical and optical yields. The simple and rapid measurement by MEKC was proved to be suitable for the kinetic resolution of recemic sulfoxide by the enzymatic reaction.
社団法人日本分析化学会の論文
1995-10-05