Salt-Stress-Responsive Membrane Proteins in Rhodobacter sphaeroides f.sp.denitrificans IL106
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概要
- 論文の詳細を見る
The salt-mediated-stress response in Rhodobacter sphaeroides f.sp.denitrificans IL 106 was investigated by culturing cells in the presence and in the absence of NaCl in growth media. Fractionation of cells followed by SDS-PAGE and 2D-PAGE revealed an increase in the levels of membrane proteins of 39 and 50 kDa and a decrease in the level of a membrane protein of 52 kDa with increasing levels of external NaCl. The proteins were isolated and sequenced. The polypeptide of 50 kDa in the inner membrane was assigned to an ATP synthase β chain and that of 52 kDa in the outer membrane to a flagellar filament protein. As the N terminal of the 39 kDa protein in the outer membrane was blocked, partial proteolysis was carried out and four peptides were sequenced. Each sequence exhibited no significant homology with those available in databases, suggesting that the polypeptide of 39 kDa (named SspA) is a novel salt-stress-induced protein.
- 社団法人日本生物工学会の論文
- 2001-02-25
著者
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大久保 明
東京大学大学院農学生命科学研究科
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Okubo Akira
Department of Applied Chemistry, The University of Tokyo
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Yamazaki Sunao
Department of Applied Chemistry, The University of Tokyo
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大久保 明
Univ. Tokyo Tokyo Jpn
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Okubo A
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Okubo Akira
Department Of Applied Biological Chemistry The University Of Tokyo
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Okubo Akira
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Yamazaki S
Faculty Of Environmental Studies Nagasaki University
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Yamazaki Sunao
Department Of Applied Biological Chemistry The University Of Tokyo
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Abo M
Univ. Tokyo Tokyo Jpn
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Abo Mitsuru
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Yamazaki Sunao
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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XU XIAOYUAN
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The U
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Xu Xiaoyuan
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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OKUBO Akira
Department of Agricultural Chemistry, The University of Tokyo
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