血清及び肝臓中のビタミンAの高速液体クロマトグラフ定量法

概要

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Two method have been developed for the determination of vitamin A in serum and liver by high-speed liquid chromatography (HSLC) using on-line spectrofluorometer. Liver in which vitamin A is present as palmitate, was homogenized and extracted with n-hexane and the n-hexane extract was evaporated under N_2 gas at 30℃. The residue was dissolved in an isopropanol solution of benzo (a) pyrene, and subjected to HSLC using Permaphase ODS column and isopropanol・ethanol・H_2O (56 : 16 : 28) as mobile phase, and vitamin A was determined by internal standard method. Serum in which vitamin A was present as alcohol, was extracted with n-hexane identically. The residue was dissolved in chloroform and subjected to HSLC using JASCOPACK WC-03 column and chloroform・isooctane (0.5 : 99.5) as mobile phase. Vitamin A was determined from calibration curves. The proposed HSLC methods were not disturbed by other fat soluble substances and the detection limits were one tenth as compared with conventional methods.
日本ビタミン学会の論文
1977-07-25