螢光検出器付高速液体クロマトグラフィーによる血清中のユビキノンの定量

概要

論文の詳細を見る
The reaction of ubiquinone (UQ) with ethylcyanoacetate (ECA) to obtain a fluorescent substance and the application of the reaction to the quantitative determination of UQ in serum by high-speed liquid chromatography (HSLC) were studied. The most sensitive and quantitative fluorescence was obtained when UQ was reacted with ECA at 50℃ for 20 hours. Since the fluorescent substance was unstable to exposure of light, the reaction should be performed in a brown tube. Serum was extracted with n-hexane in the presence of ethanol and the extract was evaporated under N_2 gas at 30℃. The residue was reacted with ECA reagent and then applied to the HSLC using Permaphase ODS column and ethanol・water (75 : 25) as a mobile phase. The peak was detected with a spectrofluorometer (Ex. 430 nm, Em. 530 nm). The proposed method was not affected by other fat-soluble substances and more specific and simple than the other conventional determination methods.
日本ビタミン学会の論文
1977-03-25