螢光反応検出を利用した高速液体クロマトグラフィーによる生体中のフィロキノン-2,3-エポキシドおよびメナキノン-4-2,3-エポキシドの定量

概要

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A high performance liquid chromatographic method was developed for the simultaneous determination of vitamin K and vitamin K oxide in biological materials. The method involves n-hexane extraction of plasma or liver homogenate and reversed phase separation on Nucleosil[○!R] C_<18> with a mobile phase of methanol-ethanol (7 : 3,v/v) followed by fluorometric detection of the reaction products with NaHSO_3-HCl and NaBH_3CN. Minimum detectable quantity was 2 ng for phylloquinone and menaquinone-4,and 3 ng for phylloquinone oxide and menaquinone-4 oxide, respectively. This method is simpler and more specific than the conventional methods for the determination of vitamin K oxide.
公益社団法人日本薬学会の論文
1979-10-25