還元糖の定量 (第4報) : BLOM變法の提唱

概要

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1) We propose the modified BLOM's method as the rapid method for the determination of the reducing sugars.2) This modified BLOM's method has been improved by decreasing the variation of the titration value by means of a special glass apparatus (Fig. 1).3) As the end point of the titration in the present method is as distinct as in BLOM's method and more distinct than in LANE's method, we expect that this mehtod will be adopted very widely.4) The procedure used for the estimation of the reductng sugars is as simple as LANE's method and BLOM's method. The proposed method, moreover, is excellent for the reasons that the sugar amount is able to be calculated by the following formnla and accordingly the sugar tables are not necessary. Sugar concentration (mg/100 ml)=F/(Titer of sugar solution in ml.). Forexample, in case of glucose, F=1541 (100×glucose factor in Table I) and other sugar factors have been shown in Table III. But the error in the present method for the estimation of glucose is ±1.6% and the determining range in this method is from 60 mg to 160 mg per 100ml as glucose.5) In the proposed method, the ratios of reducing power of other mono-saccharides to glucose are as follows : glucose 100,mannose 92,galactose 90,arabinose 95,xylose 104,fructose 106.
公益社団法人日本生物工学会の論文
1953-07-15