糖の定量に關する研究(第8報) : ニトロ化合物を用いる糖の比色定量

概要

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It is the purpose of this research to find a simple and accurate colorimetric method of sugar determination.The author has traced LEWIS-BENEDICT's method, SUMNER's method, and KINGSBURY's method for the colorimetric determination with nitro-compounds, using the spectrophoto-meter of PULFRICH photometer.In the first place, the absorption spectra of monoasccharides were examined by these three methods.Subsequently, the three methods were applied to the determination of sugar, and the following results were obtained.1) KINGSBURY's method has no reproducibility. Therefore, this method is not applicable to the determination of sugars.2) Sugars in sake and saccharified solutions of feeds were determined colorimatrically by LEWIS-BENEDICT's method and SUMNER's method, and both the methods gave a higher value than BERTRAND's method. This reason is unknown, but it was found that alcohol, succinic acid, oxalic acid, citric acid, peptone, glycine, leucine, asparagine and cystine exerted almost no influence on both the methods.
公益社団法人日本生物工学会の論文
1955-10-15