過塩素酸セリウム酸化による炭水化物のポーラログラフ分析法

概要

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A new polarographic method is described for the determination of carbohydrate. This method is based upon the stoichiometric oxidation with ceric perchlorate in strong perchloric acid.The process of this method is as follows : to 1 ml sugar solution at the level of less than 1.5mg, add 2ml 0.05M ceric perchlorate reagent, leave it for 1 hour at 60℃, reduce the excess Ce (IV) by the addition of Fe (II) solution, and add 0.2ml gelatine and water making a total of 10ml solution. Fe (III), equivalent to the residual Ce(IV), is determined by the polarographic process, by which the diffusion current of this solution (A) and sugar blank (B) vs. potassium chloride saturated mercury pool electrode are measured at zero applied potential.The amount of aldosis calculated as follows : aldose (mg)=added Ce(IV) m mole ×(mol.wt)./(2C)×(B-A)/(B-B')B' is correction of Fe (II) in Fe (II) soluton, and C signifies the number of carbon atom in aldose molecule. The second term is usualy 15.The recovery of the glucose determination rang in the process is from 99.4 to 100.5%, and the standard deviation is 0.39.
社団法人日本生物工学会の論文
1964-04-15