ブドウ糖存在下の果糖の定量

概要

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Three titration methods (PLANK's indirect and MATHEWS' methods) and three colorimetric ones (diphenylamine, crysteine-carbazol and resorcin-thiourea methods) for the determination of fructose in the presence of glucose were re-examined and compared with one another, and the following results were obtained : Of the three titration methods, the indirect method in which glucose had been determined by the hypoiodite method and fructose calculated by the difference between glucose value and total reducing value was most accurate. Otherwise, of the three colorimetric methods, the crysteine-carbazol method showed the slightest influence of the co-existing gluclose on the determination of fructose and the ratio of the absorption coefficients of fructose and glucose was 280 : 1 in that case.The values obtained as total fructose component in mandarin and persimmon by the hypoiodite method were 3.165ω/υ% and 5.076ω/υ, respectively, but those by three colorimetric methods 4.056〜4.280ω/υ% and 6.652〜7.014_ξω/υ%, respectively. The latter are about thirty per cent higher than the former. The reason for this difference will be studied in future.
公益社団法人日本生物工学会の論文
1958-08-15