フェノール・硫酸法による炭水化物の比色定量法の検討(I) : キシローズの比色定量並びに混合物中のキシローズとグルコーズの迅速分析

概要

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1) The color of the phenol H_2SO_4 reaction of xylose has the maximum absorption at 480 mμ.The optimal condition for the determination of xylose is as follows : The xylose solution (2 ml) and 0.1 ml of phenol reagent (5 g/100 ml H_2O) are mixed in a test tube. H_2SO_4(96%, 5ml) is added to the test tube along the wall, being carefully shaken in the cold water to avoid the temperature rise. Heat the test tube for 3.5 min.in a vigorously boiling water bath. After rapid cooling the color of the solution is measured by the spectrophotometer.BEER's law is obeyed satisfactorily up to 0.004% of xylose solution, and the standard deviation of the present colorimetric method is 2.55%.2) A rapid method, based on the phenol H_2SO_4 reaction, is used for the estimation of xylose and glucose in mixtures. Results are within 8% error for xylose in the concentration range of 0.4〜0.8mg% and 1.3% error for glucose in the concentration range of 3.2〜3.6 mg%.
公益社団法人日本生物工学会の論文
1958-02-15