還元糖の定量(第1報) : 葡萄糖定量4方法の實用價値比較

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With regard to four methods of glucose determination, namely the methods of BERTRAND, LANE, ISSEKUTZ-BOTH and WILLSTATTER-SCHUDEL, expense, convenience, preservability of the reagent, precision, an d influence of impurities were compared with one another, and the following results were obtained : 1) BERTRAND'S method is most expensive and inconvenient.2) With regard to the pure glucose solution, BERTRAND'S method showed the most accurate value, BOTH'S method came after it, and LANE'S method showed somewhat low value (98.42%).3) With regard to the WILLSTATTER'S method, it seems that the special caution is necessary for determinative procedure. Therefore, the authors do not give any conclusion here.4) The presence of NaCl ard ethyl alcohol exerted almost no influence upon BERTRND'S method, LANE'S method and BOTH'S method.5) In the presence of mixed solution of amino acids, BERTAND'S method and LANE'S method showed somewhat low value, BOTH'S method high value.6) In the presence of dextrin, all three methods showed high value and especially BOTH'S method the highest.7) LANE'S method was not so rought as we imagined and was much available owing to its convenience, because the influence of impurity was of the same degree as in BERTRAND'S method.8) It seems that BOTH'S method is also available, especially excellent in the point of expenses. But the influence of impurity is so great that this method may be used only for micro-determination of glucose.
公益社団法人日本生物工学会の論文
1952-08-15

還元糖の定量(第1報) : 葡萄糖定量4方法の實用價値比較

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