硫黄細菌の生化学的研究 : (第4報)菌の生育とエネルギー効率(その2)

概要

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1) The effects of oxygen gas tension on the growth rate and the free energy efficiency of growth for T. thiooxidans were studied with "sealed shaking culture" The growth rate and the efficiency reached two peaks which were maximum in the presence of 15 to 30 per cent and of 50 to 60 per cent O_2,respectively. 2) Glucose was slightly utilized by the bacterium as a carbon source for growth. Where glucose was used in place of CO_2 the growth rate was much lower than normal, but sulfur oxidation was little affected. 3) The effects of CO_2 tension on the growth rate and the free energy efficiency were studied with the "sealed shaking culture". The growth was inhibited by CO_2 at above 20 per cent level of CO_2,while the sulfur oxidation was little affected. At below 4 per cent of CO_2,the growth was affected and the efficiency was markedly decreased. It was observed that the efficiency of energy utilization varied by the ratio of 3 : 2 : 1 on the whole, in accordance with the various conditions. From these results, it may be suggested that this bacterium possesses several metabolic pathways of sulfur oxidation for producing high-energy phosphate. 4) The maximum growth was reached in about 9 days at 30℃ with using equipment as shown in Fig. 1 the yiedl was usually 0.7g dry weight per l. This value was about three times as high as the yield obtained with usual shaking culture.
社団法人日本生物工学会の論文
1965-01-25