N菌群細菌(低温好塩性ヒスタミン生成菌)のヒスタミン生成に及ぼす温度, pH およびNacl濃度の影響

概要

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Many studies have been made on hisidine decarboxlase by the mesophiles such as P. morganii, E. coli and C. perfringens, however the decarboxylation mechanism of the psychrophilic and halophillic histamine former such as the N-group bacteria has not yet been studied. The present paper deals with the effects of temperature, pH value and NaCl concertration on the histidine decarboxylation by the N-group bacteria. Results obtained are summarized as follows: (1) The optimum temperature for the growth of the N-group bacteria was around 20°C, while that for the histamine formation was about 25°C, the highest temperature for supporting their growth. The histidine decarboxylase activity of the washed cell suspension tested was highest at a temperature between 35°C to 40°C. (2)The optimum pH value for the growth of the N-group bacteria was between 6 to 8, while that for the histamine formation was be ween 5 to 6. The activity of histidine decarboxylase of the washed cell suspension was highest at pH 5.4. (3) The optimum concentration of NaCl for the histamine formation by the N-group bacteria was 1 to 3%, corresponding to the optimum concentration for their growth. On the other hand, there was no correlation observed between the histidine decarboxylase activity of the washed cell suspension and the Nacl concentration.
日本水産學會の論文