セルラーゼ生産菌Robillarda sp.の単離とその培養条件

概要

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A fungus, strain no. Y-20,which produced mainly Cx enzyme of cellulase system in the culture filtrate, was isolated from soil in Japan. The fungus was identified and the culture conditions for cellulolytic enzyme production were studied.1) The strain was identified to belong to Robillarda gen. by taxonomical characteristics.2) The culture liquid of the fungus showed high CM-cellulose saccharifying activity. The optimal medium was 2% cellulose powder, 0.2% casamino acids, 0.01% yeast extract, 0.1% K_2HPO_4,0.05% MgSO_4・7H_2O, 0.05% KCl, and 0.001% FeSO_4・7H_2O, pH 6.0 When the fungus was cultured at 30℃ for 7 days on a rotary shaker at 148 rpm in a 500-ml Erlenmeyer flask with three dents containing the optimal medium, the maximal production of microcrystalline cellulose and CM-cellulose hydrolyzing enzymes was obtained, and the activities were 0.12 and 19 units/mg protein (0.06 and 10 units/ml), respectively.3) Microcrystalline cellulose and CM-cellulose hydrolyzing enzymes of the culture filtrate were compared with those of Trichoderma reesei QM9414. The productivity of cellulase was lower than that of T.reesei QM9414,but the specific activity of CM-cellulose hydrolyzing enzyme in the culture filtrate was higher than that of T. reesei QM9414,reaching 4.9-fold. This indicates that Robillarda sp. Y-20 is a fungus which produce the cellulolytic enzyme of a high Cx enzyme activity.
社団法人日本生物工学会の論文
1985-11-25