Arsenic Induces DNA Damage via Reactive Oxygen Species in Human Cells
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概要
- 論文の詳細を見る
To elucidate arsenic-induced oxidative DNA damage, the genotoxicity of arsenic in human cells was comparatively studied with single cell gel electrophoresis (SCGE) assay in combination with the observation of the protective effects of dimethyl sulfoxide (DMSO) and catalase. Arsenic, at the concentration of 2.4μM by coincubation for 24 hours, significantly induced DNA damage in HL60, a human promyelocytic leukemia cell line. In contrast, significant DNA damage was found in human mononucleocytes at the concentration of 4.8μM or above. The cells were incubated separately with DMSO (12 mM/l), a well-known hydroxyl radical (OH^-) scavenger, and catalase (1, 300 U/ml), a hydrogen peroxide (H_2O_2) scavenger, for 6 hours and then further coincubated with various concentrations of arsenic for 24 hours at 37℃ and 5% CO_2. The findings showed that both DMSO and catalase significantly reduced the arsenic-induced tail moment, a parameter of total damaged DNA, in HL60 and mononucleocytes. Hence our findings indicate that arsenic, with micromolar concentrations, induces typical and various extents of DNA damage in human cells via reactive oxygen species in a dose-dependent manner.
- 日本衛生学会の論文
著者
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TAKESHITA Tatsuya
Department of Public Health, Wakayama Medical University
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MORIMOTO Kanehisa
Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine
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LU YUQUAN
Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University
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Takeshita T
Department Of Hygiene And Preventive Medicine Osaka University School Of Medicine
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Takeshita Tatsuya
Department Of Social And Environmental Medicine Osaka University Graduate School Of Medicine
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Takeshita Tatsuya
Ntt Opto-electronics Labolatories
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Takeshita Tatsuya
Department Of Social And Environmental Medicine Course Of Social Medicine Osaka University Graduate
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Takeshita Tatsuya
Department Of Social And Environmental Medicine Osaka University Graduate School Of Medicine F1
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Takeshita Tatsuya
Department Of Hygiene And Preventive Medicine Osaka University School Of Medicine
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Lu Yuquan
Department Of Social And Environmental Medicine Osaka University Graduate School Of Medicine
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Lu Yuquan
Department Of Social And Environmental Medicine Graduate School Of Medicine Osaka University
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Takeshita T
Department Of Social And Environmental Medicine Course Of Social Medicine Osaka University Graduate
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LI Dasheng
Department of Preventive Medicine, Osaka University Graduate School of Medicine
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Li Dasheng
Department Of Preventive Medicine Osaka University Graduate School Of Medicine
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Morimoto Kanehisa
Department Of Hygiene And Preventive Medicine Osaka University School Of Medicine
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LU Yuquan
Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine
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Takeshita Tatsuya
Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine
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TAKESHITA Tatsuya
Department of Health Sciences, Yamanashi Medical College
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