Effects of (-)-Epigallocatechin Gallate (EGCG) on DNA Strand Breaks as Evaluated by Single-cell Gel Electrophoresis (SCG) in Human Lymphocytes
スポンサーリンク
概要
- 論文の詳細を見る
(-)-Epigallocatechin gallate (EGCG), a catechin polyphenol component, is the main ingredient of green tea extract. Although the anti-carcinogenic and cancer inhibitory effects of EGCG have been widely reported, its genotoxicity is not clear and seldom reported. In this study, we examined the effects of EGCG on DNA strand breaks in the isolated lymphocytes and whole blood lymphocytes obtained from two smoking subjects and a nonsmoking healthy subject using a single-cell gel electrophoresis (SCG) assay. The results showed that after 2 hrs of treating the isolated lymphocytes from the smokers, EGCG induced a significant increase in DNA strand breaks at concentrations from 2.5×10^<-5>M to 2.0×10^<-4> M, while after 2 hrs of treating the whole blood obtained from the same smokers, EGCG suppressed the DNA strand breaks in the lymphocytes at concentrations of 1.0×10^<-4> M and 2.0×10^<-4> M, A similar suppressive result was also shown in the whole blood lymphocytes from the nonsmoker at nearly the same concentrations, while at concentrations of 1.0×10^<-3> M or 2.0×10^<-3> M, EGCG induced a significant increase in DNA strand breaks in the whole blood lymphocytes from the nonsmoker. This result suggests that EGCG is not only inhibitory against DNA strand breaks in whole blood, but also genotoxic to the isolated or whole blood lymphocytes at high concentrations. Thus, more research is needed to comprehensively assess the effects of EGCG on genetic materials.
- 日本衛生学会の論文
著者
-
TAKESHITA Tatsuya
Department of Public Health, Wakayama Medical University
-
MORIMOTO Kanehisa
Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine
-
LU YUQUAN
Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University
-
Takeshita T
Department Of Hygiene And Preventive Medicine Osaka University School Of Medicine
-
Takeshita Tatsuya
Department Of Social And Environmental Medicine Osaka University Graduate School Of Medicine
-
Takeshita Tatsuya
Ntt Opto-electronics Labolatories
-
Takeshita Tatsuya
Department Of Social And Environmental Medicine Course Of Social Medicine Osaka University Graduate
-
Takeshita Tatsuya
Department Of Social And Environmental Medicine Osaka University Graduate School Of Medicine F1
-
Takeshita Tatsuya
Department Of Hygiene And Preventive Medicine Osaka University School Of Medicine
-
Lu Yuquan
Department Of Social And Environmental Medicine Osaka University Graduate School Of Medicine
-
Lu Yuquan
Department Of Social And Environmental Medicine Graduate School Of Medicine Osaka University
-
Takeshita T
Department Of Social And Environmental Medicine Course Of Social Medicine Osaka University Graduate
-
Morimoto Kanehisa
Department Of Hygiene And Preventive Medicine Osaka University School Of Medicine
-
LU Yuquan
Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine
-
Takeshita Tatsuya
Department of Social and Environmental Medicine, Osaka University Graduate School of Medicine
-
TAKESHITA Tatsuya
Department of Health Sciences, Yamanashi Medical College
関連論文
- Upregulation of Fractalkine and Its Receptor, CX3CR1, is Associated With Coronary Plaque Rupture in Patients With Unstable Angina Pectoris
- Work and Family Life of Childrearing Women Workers in Japan : Comparison of Non-Regular Employees with Short Working Hours, Non-Regular Employees with Long Working Hours, and Regular Employees
- Effects of Work-Related Factors and Work-Family Conflict on Depression among Japanese Working Women Living with Young Children
- Increasing Obesity among Male Workers in Japan : 1992-1997
- Association of Tooth Loss with Psychosocial Factors in Male Japanese Employees
- The Analysis of Trends in Induced Abortion in Japan : An Increasing Consequence among Adolescents
- The Beneficial Effect of Gauze Mask on Methyltetrahydrophthalic Anhydride-Induced Allergic Rhinitis
- Study on Allergic Rhinitis in Workers Exposed to Methyltetrahydrophthalic Anhydride
- Oral Taurine Supplementation Prevents the Development of Ethanol-Induced Hypertension in Rats
- THE RELATIONSHIP BETWEEN DENTAL HEALTH AND SISTER CHROMATID EXCHANGE (SCE)
- Skin Sensitization Study by Quantitative Structure-Activity Relationships (QSAR)
- Long-term effects of lifestyle on multiple risk factors in male workers
- The Effect of the Weight Reduction on the Salivary Cortisol Levels of Judo Players
- The Unique Correlation between Anti-Mutagenicity of Human Saliva and Change in Body Weight
- Secretory IgA in Saliva can be a Useful Stress Marker
- Perturbation of Secretory Ig A in Saliva and Its Daily Variation by Academic Stress
- A Feeling of Interest was Associated with a Transient Increase in Salivary Immunoglobulin A Secretion in Students Attending a Lecture
- The biological effects of negatively-charged indoor air conditions
- Moderating Effects of Psychological Job Strain on the Relationship between Working Hours and Health : An Examination of White-Collar Workers Employed by a Japanese Manufacturing Company
- A Discretionary Work Scheme May Induce a Change in the Association of Working Hours and Vacations with Health Problems in Middle-Aged Male White-Collar Workers
- Work versus Non-Work Predictors of Job Satisfaction among Japanese White-Collar Workers
- An Investigation into the Effects of Vacations on the Health Status in Male White-collar Workers
- Correlation between Saliva Glycated and Blood Glycated Proteins
- Application of Specific Brain Function Evaluation by Optical Topography
- Smoking, Alcohol Drinking and Esophageal Cancer : Findings from the JACC Study
- Relationships between Cigarette Smoking, Alcohol Drinking, the ALDH2 Genotype and Adenomatous Types of Colorectal Polyps in Male Self-Defense Force Officials
- Seismic Intensity and Mental Stress after the Great Hanshin-Awaji Earthquake
- Life Events and Posttraumatic Stress in Hanshin-Awaji Earthquake Victims
- The Relationship of Lifestyle Factors, Personal Character, and Mental Health Status of Employees of a Major Japanese Electrical Manufacturer
- Effects of Shiftwork on Lifestyle and Mental Health Status of Employees of a Major Japanese Electrical Manufacturer
- Association of Smoking with Other Lifestyle Factors and Mental Health Status of Japanese Factory Workers
- The Effects of Lifestyle and Type A Behavior on the Life-Stress Process
- Risk factors for knee osteoarthritis in Japanese men : a case-control study
- Effects of Static Magnetic Fields on the Induction of Sister Chromatid Exchange in Human Lymphocytes in vitro
- The Effect of Ageing on Mutagen-Induced SCE in Mouse Spleen Lymphocytes
- The Effect of Ageing on Cell Cycle Kinetics in Mouse Spleen Lymphocytes
- X-ray induced Sister Chromatid Exchange and Chromosomal Aberrations in the Lymphocytes from the Patients with Neurofibromatosis
- Development of a Monitoring System for Human Exposure to Tritium : Chromosome Aberrations in Human Lymphocytes Exposed to HTO
- Diagnosis and Prevention of Diseases Induced by Isocyanate
- Review of the Occupational Exposure to Isocyanates : Mechanisms of Action
- Arsenic Induces DNA Damage via Reactive Oxygen Species in Human Cells
- Effects of (-)-Epigallocatechin Gallate (EGCG) on DNA Strand Breaks as Evaluated by Single-cell Gel Electrophoresis (SCG) in Human Lymphocytes
- Occupational Hypersensitivity Pneumonitis Due to Isocyanates:Mechanisms of Action and Case Reports in Japan
- N-Acetyltransferase Polymorphism and Human Cancer Risk
- P300 as a Measure of Cognitive Dysfunction from Occupational and Environmental Insults
- Prevalence of Helicobacter pylori Infection in Japan : Relation to Educational Levels and Hygienic Conditions
- Single-Cell Gel Electrophoresis (SCG)-A Review and Discussion
- A Further Note on the Sampling Device for the Anti-Mutagenicity of Saliva
- Daily Lifestyles and Anti-Mutagenicity of Saliva
- Lifestyle, Mental Health Status and Salivary Secretion Rates
- Quality of Life Domains in the Healthy Public : A Trial Investigation Using Attendants for an Annual Health Checkup
- High-Output Power and Fundamental Transverse Mode InGaAs/GaAs Strained-Layer Laser with Ridge Waveguide Structure
- Low-Threshold Strained-Layer InGaAs Ridge Waveguide Lasers
- Effects of ECR Hydrogen-Plasma Treatment on Narrow-Stripe Polycrystalline Silicon Thin-Film Transistors
- Study of ECR Hydrogen Plasma Treatment on Poly-Si Thin Film Transistors : Semiconductors and Semiconductor Devices
- Study on Narrow-Stripe Polycrystalline Silicon Thin-Film Transistors : Semiconductors and Semiconductor Devices
- Relations Among TMD, Bruxism, Lifestyle, and Psychological Stress
- Effect of gene polymorphisms and ethanol consumption on micronucleus frequency in human reticulocytes : a preliminary study
- Beneficial Effect of Tomato Juice Drinking on Anti-Mutagenicity of Saliva
- Lifestyles and Psychosomatic Symptoms among Elementary School Students and Junior High School Students
- Health Practice in Islam : The Cultural Dependence of the Lifestyle Formation
- Cigarette Smoking is Associated with the Reduction of Lymphokine-activated Killer Cell and Natural Killer Cell Activities
- Acute Caffeine Effect on Repeatedly Measured P300
- Effects of Genetic Polymorphisms in Alcohol-metabolizing Enzymes on Alcohol Hypersensitivity and Alcohol-related Health Problems in Orientals
- Prevention of Occupational Allergy Caused by Exposure to Acid Anhydrides
- Gain Characteristics of Optical-Gate Switches Using InGaAs/GaAs Strained-Layer Quantum Welts
- Categorization of Micronuclei by Size and Measurement of Each Ratio in Cytokinesis-block and Conventional Cultures of Human Lymphocytes exposed to Mitomycin C and Colchicine
- Effect of Snack Eating on Sensitive Salivary Stress Markers Cortisol and Chromogranin A
- Genotype Difference of Aldehyde Dehydrogenase 2 Gene in Alcohol Drinkers Influences the Incidence of Japanese Colorectal Cancer Patients
- The modulation of immunological activities in human NK cells by extracts of ginkgo
- HEALTH PROMOTION ACTIVITIES IN JAPAN
- Correlation between Molecular Weight of Plasma Albumin Determined with Mass Spectrometry and Glycation Indices in Factory Workers
- Levels of awakening salivary CgA in response to stress in healthy subjects
- Assessment of accuracy of immediate blood separation method : a novel blood analysis strategy
- Influence of personal patterns of behavior on the effects of Tai Chi : a pilot study
- A respiratory sensitization study by a new quantitative structure-activity relationships (QSAR)
- Does the Japanese Society for Hygiene need its own Code of Conduct? A comparison of the responses of councilors and junior members based on a questionnaire survey
- An Investigation of the Effects of Lifestyle on Care-seeking Behavior Using Data from Health Insurance Claims
- Effects of Mental Arithmetic Stress on Blood Cell Counts and the Immune System
- Relationship Between Coffee Consumption and Prevalence of Metabolic Syndrome Among Japanese Civil Servants
- Frequencies of sister chromatid exchanges in cells from patients with neurofibromatosis induced by mitomycin-C.
- Vinyl acetate-induced sister chromatid exchanges in murine bone marrow cells.
- Spontaneous and mitomycin-C induced sister chromatid exchanges in cells from patients with tuberous sclerosis.
- P-013 An Improved Method for Detecting DNA Repair by Using Alkaline Comet Assay(Poster presentation)
- Gain Characteristics of Optical-Gate Switches Using InGaAs/GaAs Strained-Layer Quantum Wells
- Epidemiologic Features and Prognostic Factors of Coronary Artery Lesions Associated With Kawasaki Disease Based on a 13-Year Cohort of Consecutive Cases Identified by Complete Enumeration Surveys in Wakayama, Japan