Enhanced Secretion of Peroxidase from Carrot Hairy Roots Using Polyethylene Glycol
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概要
- 論文の詳細を見る
Effects of various organic solvents and polyethylene glycol (PEG) on the secretion of peroxidase from carrot hairy roots were investigated. The peroxidase activity in culture broth was significantly enhanced without adverse effects on root growth when PEG was employed while other organic permeabilizing agents exerted harmful effects on root growth. PEG with a molecular weight of over 6,000 g/mol enhanced the secretion of peroxidase dramatically. Since PEG did not induce the formation of pores in the root cell membrane as confirmed by staining using Neutral Red, it was thought that the mechanism of secretion enhancement of peroxidase by PEG is different from that of other organic solvents which make the cell membrane permeable. Since PEG does not adversely affect the root growth, PEG can be applied to the repeated use of root cells for production of peroxidase.
- 公益社団法人日本生物工学会の論文
- 1997-04-25
著者
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Yoo Young
Department of Anesthesiology and Pain Medicine, Yonsei University Health System
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Kim Ji
Department Of Anesthesiology And Pain Medicine Gachon Medical School Gil Medical Center
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Kim Y
Seoul National Univ.
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Kim Y
Department Of Chemical Engineering And The Institute Of Genetics And Molecular Biology Seoul Nationa
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Kim Yong
Department And Research Institute Of Rehabilitation Medicine Yonsei University College Of Medicine
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Yoo Young
Department Of Anesthesiology And Pain Medicine Yonsei University Health System
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Yoo Young
Department Of Chemical Engineering And The Institute Of Genetics And Molecular Biology Seoul Nationa
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Kim Ji
Department Of Chemical Engineering And The Institute Of Genetics And Molecular Biology Seoul Nationa
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Kim Ji
Department of Advanced Materials Engineering, Sangju National University, Kyeongbuk 742-711, Korea
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Yoo Young
Department of Advanced Materials Engineering, Yonsei University, Seoul 120-749, Republic of Korea
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