Restriction Endonuclease Map of the Root-inducing Plasmid (pRi1724) of Agrobacterium rhizogenes Strain MAFF03-01724
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概要
- 論文の詳細を見る
The Agrobacterium rhizogenes strain 1724 isolated from diseased melon plants in Japan [Shiomi et al. , Ann. Phytopath. Soc. Jpn. , 53, 454-459 (1987)] carries a mikimopine-type root-inducing plasmid with 215-kb size (pRi1724). This plasmid DNA has been partially digested with the restriction endonuclease BamHI, and the resulting DNA fragments cloned in a plasmid vector (pBR329) and a cosmid vector (pHC79)for a pRi1724 gene library. DNA inserted in each clone was identified by complete cutting with BamHI and subsequent agarose gel electrophoresis. The physical order of BamHI fragments was deduced from a series of overlapping clones, creating a circular map. A virulence region was located on the map by Southern blot hybridization toward those of the agropine-type plasmid pRiA4b.
- 社団法人日本農芸化学会の論文
- 1994-02-23
著者
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Tanaka N
Department Of Applied Biological Sciences Faculty Of Agriculture Saga University
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OKA Atsuhiro
Laboratory of Molecular Genetics, Institute for Chemical Research, Kyoto University
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Oka Atsuhiro
Laboratory Of Molecular Biology Institute For Chemical Research Kyoto University
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Oka Atsuhiro
Laboratory Of Molecular Genetics Institute For Chemical Research Kyoto University
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Tanaka N
Jojinkai Hospitals And Welfare Institutes
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Tanaka Nobukazu
Bioassay Laboratory, Research Center, Daicel Chemical Industries, Ltd.
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Tanaka Nobukazu
Bioassay Laboratory Research Center Daicel Chemicallndustries Ltd.
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