A Transformation System for an Ectomycorrhizal Basidiomycete, Lyophyllum shimeji(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
A transformation vector, pLS-hph, was constructed with the promoter and terminator of the glyceraldehyde-3-phosphate dehydrogenase (GPD) gene derived from an ectomycorrhizal basidiomycete, Lyophyllum shimeji, and with the hygromycin B (HmB) phosphotransferase (hph) gene from Escherichia coli. This vector was introduced into protoplasts of L. shimeji and 3.4 transformants per μg plasmid DNA were obtained. In most of the transformants, multiple copies of the vector were integrated into the genomic DNA. The results indicate that pLS-hph is a useful vector for L. shimeji.
- 社団法人日本農芸化学会の論文
- 2001-08-23
著者
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Saito Tetsuya
Department Of Biological And Chemical Engineering Faculty Of Engineering Gunma University:the Mushro
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Tanaka N
Jojinkai Hospitals And Welfare Institutes
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TANAKA Norio
The Mushroom Research Institute of Japan
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SAITO Takeshi
Depertment of Biological and Chemical Engineering, Faculty of Engineering, Gunma University
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SHINOZAWA Takao
Depertment of Biological and Chemical Engineering, Faculty of Engineering, Gunma University
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Shinozawa T
Gunma Univ. Gunma Jpn
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