Characterization of Subrepeat Regions within rDNA Intergenic Spacers of the Edible Basidiomycete Lentinula edodes(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
For 16 commercial cultivars of Lentinula edodes, DNA fragments for the nuclear rDNA intergenic spacers IGS1 and IGS2 were amplified and analyzed. IGS1 contained a subrepeat region, named SR1, and IGS2 contained a pair of direct repeats and a subrepeat region, named SR2. Three and five types of subrepeats were found in SR1 and SR2, respectively. Heterogeneity in the lengths of IGS1 and IGS2 arose mainly from the number of different kinds of subrepeats within SR1 and SR2. The DNA fingerprints from the PCR products targeting SR1 and SR2 were specific for each of the 16 cultivars, and had enough variation for discrimination among the cultivars. This result suggests that the DNA fingerprints targeting SR1 and SR2 are useful for investigations of L. edodes cultivars.
- 社団法人日本農芸化学会の論文
- 2002-10-23
著者
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SAITO Takeshi
Department of Surgery, Funabashi Municipal Medical Center
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SHINOZAWA Takao
Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University
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Saito Takeshi
Department Of Forensic Medicine Tokai University School Of Medicine
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Saito Tetsuya
Department Of Biological And Chemical Engineering Faculty Of Engineering Gunma University:the Mushro
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Tanaka N
Jojinkai Hospitals And Welfare Institutes
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TANAKA Norio
The Mushroom Research Institute of Japan
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Shinozawa T
Gunma Univ. Gunma Jpn
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Shinozawa Takao
Department Of Biological And Chemical Engineering Faculty Of Engineering Gunma University
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Saito Takeshi
Department Of Cardiovascular Surgery Sendai Open Hospital
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Saito Takeshi
Department of Applied Physics, Hokkaido University, Kita-13, Nishi-8, Kita-ku, Sapporo 060, Japan
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