Substrate Specificity of Rat Plasma Lecithin-cholesterol Acyltransferase towards a Molecular Species of Phosphatidylcholine

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概要

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• The substrate specificity and the affinity of rat purified lecithin-cholesterol acyltransferase towards the molecular species of phosphatidylcholine (PC) were studied in comparison with the human enzyme. The substrate vesicles were prepared with 40% of a test PC, 60% of egg yolk PC, and tritium cholesterol. Both human and rat enzymes showed similar high reactivity to the substrates containing three major PCs (16 : 0-18 : 1 PC, 16 : 0-18 : 2 PC, and 18 : 0-18 : 1 PC) of egg yolk compared with the vesicles of egg yolk PC alone. In the case of 18 : 0-20 : 4 PC, the rat enzyme had the highest activity among all the test PCs, but the human enzyme only had a moderate activity. Even when the substrate consisted of 18 : 0-20 : 4 PC alone, the rat enzyme had a high activity, but the activity of the human enzyme was not detected. Symmetrical diacyl-PCs (18 : 2-18 : 2 PC, 18 : 1-18 : 1 PC, 18 : 0-18 : 0 PC) were not a preferable substrate for either enzyme. The transfer of both the human and rat enzymes from the vesicles containing 18 : 0-20 : 4 PC to the egg yolk PC vesicles was on a higher level than that from the vesicles containing 18 : 2-18 : 2 PC. This suggests that the activity of the LCAT can be easily influenced by the kinds of PC molecular species and its relative content in the substrate and that the substrate may provide the extent of the enzyme transfer between the substrate particles.

• 社団法人日本農芸化学会の論文
• 1993-07-23

著者

• FURUKAWA Yuji

  Laboratory of Nutrition, Department of Science of Food Function and Health, Graduate School of Agric

• Kim H

  Ewha Womans Univ. Seoul Kor

• Furukawa Y

  Kobe Univ. Kobe

• Furukawa Yuji

  Laboratory Of Nutrition Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku Uni

• Kimura Shuichi

  Laboratory Of Nutrition Graduate School Of Agricultural Science Tohoku University

• Kimura S

  Tohoku Univ. Sendai Jpn

• Kimura S

  Department Of Microbiology Kochi Medical School

• Urano Takashi

  Laboratory Of Nutrition Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku Uni

• Hida Yoshifumi

  Laboratory Of Nutrition Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku Uni

• Hida Yoshifumi

  Laboratory Of Nutrition Chemistry Division Of Applied Life Sciences Graduate School Of Agriculture K

• Kim Hyoun

  Laboratory of Nutrition, Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku

• Kimura Shuichi

  Laboratory Of Nutrition Faculty Of Agriculture Tohoku University

• KIMURA Shuichi

  Laboratory of Nutrition Chemistry, Department of Food Chemistry, Faculty of Agriculture, Tohoku University

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