A Simple Enzymatic Quantitative Analysis of Triglycerides in Tissues
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概要
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We determined a method to measure the triglyceride levels in tissues by using a modified enzymatic kit. This enzymatic kit was originally designed to be used to measure the triglyceride levels in plasma. Our method of triglyceride level determination includes dissolving the tissue lipid extracts in an alcohol. Before using the enzymatic kit directly, the lipids were dissolved in tent-butyl alcohol, then a Triton X-100/methyl alcohol mixture was added (1/1 by volume). The presence of organic surfactants such as tent-butyl alcohol and methyl alcohol, and of a surfactant such as Triton X-100, did not interfere with the enzymatic activity. This method enabled us to determine triglyceride levels between 10 and 90 nmol, by using a spectrophotometer to measure the absorbances.
著者
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Furukawa Yuji
Laboratory Of Nutrition Department Of Applied Biological Chemistry Faculty Of Agriculture Tohoku Uni
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FURUKAWA Yuji
Laboratory of Nutrition, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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KIMURA Shuichi
Laboratory of Nutrition Chemistry, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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DANNO Hiroshi
Laboratory of Nutrition, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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JINCHO Yuu
Laboratory of Nutrition, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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BUDIYANTO Slamet
Laboratory of Nutrition, Department of Food Chemistry, Faculty of Agriculture, Tohoku University
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