1.シリカゲルによるキニンの濃縮法(第4回プラスマキニン研究会)

概要

論文の詳細を見る
Investigation of selective adsorvents for kinins from bilogical materials was carried out. Inorganic polymer consisting of silicic acid, such as silica gel florisil, kaolin or acidic terra abla was a suitable adsorbent for kinin condensation. Among them, silica gel was the best one which adsorbed kinins tightly from aqueous or alcholic solution while albumin, acidic or neutral amino acids, urea or salts were not adsorbed. The kinins on silica gel was easily eluated with the mixture of acetic and pyridin. Recovery rate of added 50 ng of bradykinin in body fluids was 40-80%. The method was applied for the analysis of urinary kinins plasma kinins or kinins in amniotic fluid or lymph. The values obtained were 10-20 ng/ml (human urine), 5-10 ng/ml (human amniotic fluid) and below 1 ng/ml (human plasma and dog lymph). Kinin fraction from human urine was further purified by ion-exchange chromatography. Bradykinin and kallidin were identified by DNS-method.
日本アレルギー学会の論文
1970-08-30