The Quality of DNA Recovered from the Archival Tissues of Atomic Bomb Survivors is Good Enough for the Single Nucleotide Polymorphism Analysis in Spite of the Decade-long Preservation in Formalin
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概要
- 論文の詳細を見る
It is well known that the yield of DNA recovered form tissues preserved in formalin is inversely proportional to the stored duration. How is the quality? We tested the quality of DNA from archival tissues of atomic-bomb survivors stored in formalin for decades with the parameters of gene amplification efficiency by a polymerase chain reaction. All of the DNA extracted from the tissues preserved in formalin for 30 years amplified the 54-and 61-base pairs of the DNA fragments successfully. The direct sequencing of the PCR products confirmed the accurate amplification of the target sequence. A further trial to amplify the longer sequence of 111 base pairs succeeded in 20% of the samples tested. From these results, we propose a new utility of archival samples for the analysis of single nucleotide sequence polymorphism of genes, no matter how long the samples have been preserved in formalin.
- 日本放射線影響学会の論文
著者
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Nitta 1
International Radiation Information Center
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Nitta Yumiko
International Radiation Information Center Hiroshima University
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MASUDA Yuji
Department of Advanced Material Science, Faculty of Engineering, Kagawa University
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Hoshi 1
International Radiation Information Center
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TANAKA 2
Department of Hematology and Oncology, Research Institute for Radiation Biology and Medicine, Hirosh
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MASUDA 3
Department of Developmental Biology and Oncology, Research Institute for Radiation Biology and Medic
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Tanaka 2
Department Of Hematology And Oncology Research Institute For Radiation Biology And Medicine Hiroshim
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Tanaka Hideo
Department Of Applied Chemistry Faculty Of Engineering Okayama University
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Masuda 3
Department Of Developmental Biology And Oncology Research Institute For Radiation Biology And Medicine Hiroshima University
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