Sensitivity to Ionizing Radiation in Saos-2 Cells Transfected with Mutant p53 Genes Depends on the Mutation Position
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概要
- 論文の詳細を見る
We have constructed an in vitro system to examine how p53 mutants affect radiosensitivity. Mutations of p53 were made using in vitro mutagenesis, and mutant cDNAs were introduced into the human osteosarcoma cell line, Saos-2, which is devoid of endogenous p53. For wild type p5 both the expression plasmid and a regulation plasmid (LacSwitch system) were transfected into the cells. The radiosensitivities of clones of mutant p53 and wild type p53 were examined. Transformants of wild type p53 had increased radiosensitivity. The induction of wild type p53 protein by addition of IPTG did not significantly increase radiosensitivity. A mutation at codon 123 also increased radiosensitivity. Mutations at codons 143, 175, and 273 did not alter radiosensitivity.
- 日本放射線影響学会の論文
著者
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OKUMURA Yutaka
Department of Radiation Biophysics, atomic Disease Institute, Nagasaki University School of Medicine
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Ihara Makoto
Department of Applied Biological Chemistry, School of Agriculture, Kinki University
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Wang Li-hong
Department Of Radiation Biophysics Radiation Effect Research Unit Atomic Bomb Disease Institute Naga
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OKAICHI Kumio
Department of Radiation Biophysics, Nagasaki University Graduate School of Biomedical Sciences
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Okaichi Kumio
Department Of Radiation Biophysics Atomic Bomb Disease Institute Nagasaki University Graduate School
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Okaichi K
Nagasaki Univ. Graduate School Of Biomedical Sci. Nagasaki Jpn
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Okaichi Kumio
Department Of Radiation Biophysics Radiation Effect Research Unit Atomic Bomb Disease Institute Naga
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Okaichi Kumio
長崎大学医学部附属原爆後障害医療研究施設 腫瘍･診断病理学
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Ihara Makoto
Department Of Radiation Biophysics Radiation Effect Research Unit Atomic Bomb Disease Institute Naga
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Ihara Makoto
Dept. Of Radiat. Biophys. Nagasaki Univ. Sch. Med.
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Ihara M
Fujitsu Lab. Ltd. Atsugi
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Ihara Makoto
Department Of Applied Biological Chemistry School Of Agriculture Kinki University
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Okumura Y
Department Of Radiation Biophysics Radiation Effect Research Unit Atomic Bomb Disease Institute Naga
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Okumura Yutaka
Radiation Biophysics Atomic Bomb Sisease Inst. Nagasaki Univ.
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Ihara Makoto
Department Of Radiation Biophysics Atomic Disease Institute Nagasaki Univ. School Of Med.
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Okumura Yutaka
Department Of Biostatistics Scientific Data Center For The Atomic Bomb Disaster Nagasaki University
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Okaichi Kumio
Department Of Radiation Biophysics Atomic Bomb Disease Institute
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WANG Li-Hong
Department of Hematology, Peking University First Hospital
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