[日本農薬]学会賞受賞論文 殺虫剤のリガンド作動性イオンチャネルに対する作用機構研究
スポンサーリンク
概要
- 論文の詳細を見る
Ligand-gated ion channels (LGICs) mediate fast synaptic neurotransmission and are important targets for insecticides. Thus, the actions of several insecticides have been explored in electrophysiological studies on recombinant and native insect neuronal LGICs. I have shown that non-competitive antagonists of γ-aminobutyric acid gated Cl^- channels also act on glutamate-gated Cl^- channels, albeit at higher concentrations. Neonicotinoids are more potent agonists on recombinant hybrid nicotinic acetylcholine receptors (nAChRs) consisting of Drosophila Dα2 and vertebrate β2 subunits than those consisting of only vertebrate nAChR subunits (α4β2). Using this hybrid nAChR, clothianidin and related compounds containing a acyclic guanidine moiety were found to be super-agonists. Similar super-agonist actions of neonicotinoids were also observed on cultured Drosophila cholinergic neurons. Single channel nAChR recordings show that a clothianidin analogue induces a high conductance state in channel opening more frequently than acetylcholine, thereby offering a possible explanation for its super-agonist action. Unlike the case for clothianidin, imidacloprid attenuates the acetylcholine-induced response of native neuronal nAChRs when co-applied with ACh. These new discoveries add to our understanding of both the selectivity and the diverse actions of insecticides targeting LGICs.
- 日本農薬学会の論文
- 2007-08-20
著者
-
伊原 誠
Riken Spring-8 Center
-
伊原 誠
近畿大学農学部
-
Ihara Makoto
RIKEN SPring-8 Center
-
Ihara Makoto
School Of Agriculture Kinki University:(present Office)riken Harima Institute Spring-8 Center
-
Ihara Makoto
Graduate School Of Agriculture Kinki Uni.
-
Ihara Makoto
Department Of Applied Biological Chemistry School Of Agriculture Kinki University
-
伊原 誠
School of Agriculture, Kinki University
関連論文
- 1P-236 ネオニコチノイド-アセチルコリン結合タンパク質複合体の結晶構造解析(化学受容,第46回日本生物物理学会年会)
- アセチルコリンによって誘起されるα4β2ニコチン性アセチルコリン受容体応答に対するネオニコチノイドの増強および抑制効果
- イミダクロプリド, クロチアニジンおよび関連するネオニコチノイドのワモンゴキブリの神経細胞に存在するニコチン性アセチルコリン受容体に対する作用と殺虫活性との関係
- A201 イミダクロプリドのプロドラッグ設計と薬理的挙動
- 殺虫剤のリガンド作動性イオンチャネルに対する作用機構研究(学会賞受賞論文(奨励賞))
- AL1 殺虫剤のリガンド作動性イオンチャネルに対する作用機構に関する研究
- B206 イエバエ抑制性グルタミン酸受容体のC1チャネル阻害剤に対する感受性に影響を及ぼすイオンチャネル部のアミノ酸置換
- D101 ショウジョウバエKenyon細胞に対するネオニコチノイドの作用
- C208 培養昆虫神経細胞に存在するイオンチャネル類の応答のpatch-clamp法による記録
- B317 イミダクロプリドのイミノ部およびピリジン部の構造変化によるニコチン性アセチルコリンレセプターに対する作用機構の変化
- C119 ネオニコチノイド殺虫剤との相互作用におけるα7 ニコチン性アセチルコリンレセプターの loop D の役割
- Potentiating and blocking actions of neonicotinoids on the response to acetylcholine of the neuronal α4β2 nicotinic acetylcholine receptor
- Neonicotinoids Show Selective and Diverse Actions on Their Nicotinic Receptor Targets: Electrophysiology, Molecular Biology, and Receptor Modeling Studies
- Super Agonist Actions of Clothianidin and Related Compounds on the SADβ2 Nicotinic Acetylcholine Receptor Expressed in Xenopus laevis Oocytes
- Insecticidal and Neural Activities of Candidate Photoaffinity Probes for Neonicotinoid Binding Sites(Organic Chemistry)
- Sensitivity to Ionizing Radiation in Saos-2 Cells Transfected with Mutant p53 Genes Depends on the Mutation Position
- Mutation-points of p53 affect the sensitivity of the cell to anticancer drugs.
- The sensitivity to ionizing radiation and radiation-induced apoptosis in Saos-2 cells transfected by four mutants and wild typc p53.
- 1P372 Utilization of synthetic fluorescent agonist of nAChR for simultaneous optical and electrical single molecule measurements(14. Ion channels and receptors,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- The phosphatidylinositol 3-kinase, inhibited by low dose of wortmannin, affects radiation sensitivity
- Isolation and identification of histamine-release inhibitors from Pistacia weinmannifolia J. Pisson ex. Franch
- A mutant human p53, 123A, which led to apoptosis but not Waf-1 in response to ionizing radiation in a human osteosarcoma cell line, Saos-2
- Experimental Studies on MHD-Stability of an Axisymmetric Mirror with a Single Internal Hoop
- 3P097 膜タンパク質の結晶化試料スクリーニングに有用な新規Native-PAGE法の開発(蛋白質-計測・解析の方法論,第48回日本生物物理学会年会)
- [日本農薬]学会賞受賞論文 殺虫剤のリガンド作動性イオンチャネルに対する作用機構研究
- Thermosensitivity and Thermotolerance of Scid Mouse Lung Fibroblast Cells.
- 3I1322 GFP融合技術を利用した昆虫培養細胞による組換えタンパク質分泌発現の評価系(3I 蛋白質_計測・解析の方法論2,日本生物物理学会第49回年会)