63. トウモロコシ幼葉鞘の光屈性反応におけるβ-グルコシダーゼの役割

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We have hitherto reported that phototropism is caused by the unequal lateral distribution of light-induced growth inhibitor(s) in the illuminated and shaded sides, affecting the auxin activity at unchanged, even auxin level. Blue light-induced growth inhibitors were isolated as candidates for phototropism-regulating substances from maize (Zea mays L.) coleoptiles, and identified as DIMBOA and MBOA. In our previuos study, it was suggested that phototropic stimulation induces conversion of the inactive DIMBOA glucoside into the active DIMBOA, which plays the essential role in the phototropism of maize coleoptiles, and then metabolizes into MBOA, which has a lower activity. In the present study, the roles of β-glucosidase, involved in the conversion of the DIMBOA glucoside into the active DIMBOA, in maize phototropism were investigated. The net activity in the illuminated halves was significantly enhanced within 30 min after onset of unilateral blue light-illumination and then decreased, whereas the activity in the shaded halves was almost identical to those in the dark controls. These results suggested that phototropic stimulation induces its activity in the illuminated side, leading to the production of DIMBOA in a lateral gradient in the tissue. Using tissue printing technique, the distribution of β-glucosidase in the tissue was also demonstrated. The full reacting tissue printing and CBB staining revealed that an intense activity was detected in the peripheral region, which seems to be an important tissue for phototropic curvature. In addition, the effect of β-glucosidase inhibitors on the phototropic response is currently in progress. A plausible mechanism of phototropism in maize coleoptiles is presented.
植物化学調節学会の論文
2003-10-10

63. トウモロコシ幼葉鞘の光屈性反応におけるβ-グルコシダーゼの役割

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