35. アベナ発芽種子から放出される促進的アレロパシー物質

概要

論文の詳細を見る
【Object】 Molisch (1937) coined the term "allelopathy" to refer biochemical interactions between plants including microorganisms. Since then, various chemical agents involved in allelopathy have been isolated and identified from different adult plants. However, information on allelopathy during seed germination is limited. We have hitherto reported on the stimulatory allelopathy during the seed germination stage. As the stimulatory allelochemicals, lepidimoide was isolated and identified from the exudates of cress seeds, and arctigenin and arctigenic acid from those of burdock seeds, respectively. In the present study, we report on the isolation and identification of stimulatory allelochemicals exuded from germinating oat seeds. 【Methods】 We got the exudates of oat seeds by sowing them in water. The culture solution was purified by Sep-Pak cartridge chromatography and HPLC. Biological activities were tested using the bioassays of cockscomb and lettuce hypocotyls growth. The isolated stimulatory allelochemicals were identified by NMR and MS. 【Results】 The exudates of oat seeds were separated into 0, 20, 40, 60, 80 and 100% methanol in water by C_<18> Sep-Pak. The 0 and 20% methanol fractions showed the strong growth-promoting activity. The 20% fraction was subjected to HPLC (ODS). A peak (Rt. 10-12.5min) that shows growth-promoting activity were isolated and its identification is in progress.
植物化学調節学会の論文
2005-10-13