Hybridization Independent Increase of Fluorescence in a 5' Nuclease PCR Assay
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概要
- 論文の詳細を見る
In a PCR-based 5' nuclease assay with a fluorogenic 16S rDNA probe, we found an increase in fluorescence that was not caused by the digestion of the DNA probe in the PCR. The fluorescence diminished to a base line level by ethanol precipitation of the 5' nuclease PCR product. Boiling the PCR product also decreased the fluorescence to the level of a control containing no template DNA. Hence, the increase in fluorescence in the assay did not seem to be caused by hybridization to the complementary structure of the template DNA, but seemed to be dependent on the intact probe remaining in the PCR reaction mixture. The increase in fluorescence appeared to be the result of a probe/template association other than the hybridization.
- 日本微生物生態学会の論文
著者
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SASAKI Makoto
Department of Radiology, Iwate Medical University
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Ishida Yuzaburo
Labratory Of Marine Microbiology Department Of Applied Bioscience Graduate School Of Agriculture Kyo
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Sasaki Makoto
Department Of Gastroenterology And Metabolism Nagoya City University Graduate School Of Medical Scie
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Yoshinaga I
Division Of Applied Biosciences Graduate School Of Agriculture Kyoto University
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Sasaki M
Department Of Food Science And Technology National Fisheries University
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MAEDA TOSHIMICHI
Department of Food Science and Technology, National Fisheries University
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SHIBA TSUNEO
Department of Food Science and Technology, National Fisheries University
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SASAKI Makoto
Insititute for Cosmic Ray Research, University of Tokyo
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YOU BOK
Department of Food Chemistry, National Fisheries University
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YOSHINAGA IKUO
Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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Maeda Toshimichi
Natl Fish Univ
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Tsuneo Shiba
Natl Fish Univ
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Maeda Toshimichi
Department Of Food Science And Technology National Fisheries University
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Sasaki Makoto
Department Of Biochemistry Ii Nagoya City University Medical School
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Sasaki Makoto
Deparment Of Mathematical System Science Faculty Of Science And Technology Hirosaki University
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Sasaki Makoto
Department of Food Science and Technology, National Fisheries University
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