EXPRESSION OF p21 mRNA AFTER TRANSIENT FOCAL CEREBRAL ISCHEMIA

概要

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The p21 is a ubiquitous inhibitor of cyclin-dependent kinase. We examined p21 mRNA expression with in situ hybridization in a rat transient focal ischemia for 2 h. Upregulation of p21 mRNA expression started at 3 h after recirculation, when hsp 70 mRNA expression was upregulated in high level. By 12 h, p21 mRNA expression reached to the maximum level at the cortex, caudoputamen, striatum and thalamus, where glyceroaldehyde 3-phosphate dehydrogenase (GAPDH) mRNA expression was suppressed because of ischemic stress. At 24 h and 48 h, expression of p21 mRNA persisted in some parts of these regions. The p21 protein was detected with Western blotting and found upregulation with peak at 24 h. Expression of intrinsic p21 mRNA and protein in the ischemic tissue might affect cell cycle arrest and influence cell death or tissue recovery from ischemia.
名古屋市立大学の論文
2000-07-01