SUBARACHNOID HEMORRHAGE INDUCES APOPTOSIS-RELATED GENES IN THE RAT BRAIN

概要

論文の詳細を見る
We studied mRNA expression of apoptosis-related genes in the rat model of subarachnoid hemorrhage (SAH), which was induced by endovascular perforation. The TUNEL method indicated apoptotic changes in CA1 neurons of the perforated side 48 hr after SAH. The mRNA of bax, bcl-2,bcl-x and ice was induced only in the pyramidal layer and dentate gyrus of the perforated-side hippocampus. The apoptosis-regulated bax, bcl-2 and bcl-x genes were upregulated in the CA1 with peak level at 12 hr. Expression of those genes, however, varies in the CA3. The bcl-2 gene was upregulated in the CA3 with the peak level at 12 hr to 24 hr, whereas expression of bax gene was decreased rapidly after reaching peak level at 6 hr. The bcl-x mRNA was not expressed in the CA3. The mRNA of ice, apoptosis executed gene, was induced in the CA1 with peak level at 12 hr, but little expression was identified in the CA3. Sustained expression of bax at CA1 might relate to apoptotic cell death, whereas rapid reduction of bax in the CA3 may avoid neuronal death at CA3. The bcl-x may be an alternative variant of bax and may influence apoptotic process. Apoptosis-like changes in the hippocampus might correlate with neurologic dysfunction after subarachnoid hemorrhage.
名古屋市立大学の論文
2000-07-01