MyoD and myogenin mRNA levels after single session of treadmill exercise in rat skeletal muscle
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概要
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[Purpose]: We examined mRNA levels of MyoD and myogenin in rat skeletal muscle after a single session of treadmill running. [Subject]: A total of 26 male Sprague-Dawley rats aged 4 weeks were used in this study. [Methods]: Rats were run on a 16° decline for 30 min. Treadmill speeds were 0, 16, 20, 24 and 28 m/min. At 72 h post-exercise, soleus (SOL) and extensor digitorum longus (EDL) muscles were extracted. The expression of MyoD and myogenin MRNA were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and quantitative RT-PCR. [Results]: MyoD and myogenin mRNA expression were detected in all groups. Significant differences in myogenin levels were apparent in SOL between 16 m/min and 28 m/min. MyoD levels in SOL at 16, 24, 28 m/min were lower, while myogenin levels at 20, 24 and 28 m/min were higher, when compared with the 0 m/min group, although significant differences were not seen. [Conclusion]: These results suggest that a single session of exercise has little effect on proliferation of satellite cells, or myotube production. Copyright (c) 2009 by the Society of Physical Therapy Science.
- 2009-02-28
著者
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TANAKA SHOJI
Department of Impairment Study, Graduate Course of Rehabilitation Science, Division of Health Scienc
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TACHINO KATSUHIKO
Department of Impairment Study, Graduate Course of Rehabilitation Science, Division of Health Scienc
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Tanaka Shoji
Department Of Impairment Study Graduate Course Of Rehabilitation Science Division Of Health Sciences
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MIYATA TAKUYA
Department of Rehabilitation, Tsurugi Hospital
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Miyata Takuya
Department Of Rehabilitation Tsurugi Hospital
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Tanaka Shoji
Department Of Applied Physics Faculty Of Engineering University Of Tokyo
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Tachino Katsuhiko
Department Of Impairment Study Graduate Course Of Rehabilitation Science Division Of Health Sciences
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Tanaka Shoji
Department Of Applied Physics Faculty Fo Engineering University Of Tokyo
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