Post-biotinylation of Photocrosslinking by Staudinger-Bertozzi Ligation of Preinstalled Alkylazide Tag

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概要

• 論文の詳細を見る

• Post-biotinylation of the alkyl azide derivative of trifluoromethyl phenyldiazirine (TPD) was elucidated to apply a photoaffinity biotinylation technique. A photo-modified polyvinilidene difluoride (PVDF) membrane was used as a photolabeled component and we introduced biotin by Staudinger-Bertozzi ligation. The 15pmol amount of biotinylated reagent was still effective for the visualization of cross-linked product on the matrix. The results show the potential utility of alkyl azide carrying TPD derivatives in the application of photoaffinity biotinylation, which could be useful for the ligands with tight structural requirements.

• 公益社団法人日本薬学会の論文
• 2005-11-01

著者

• 橋本 誠

  北海道大学大学院農学研究院

• HASHIMOTO Makoto

  Department of Dermatology, Asahikawa Medical College

• HATANAKA YASUMARU

  Faculty of Pharmaceutical Sciences, Hokkaido University

• Hatanaka Yasumaru

  Faculty Of Pharmaceutical Sciences Hokkaido University

• Hatanaka Yasumaru

  Fac. Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical Univ.

• Hashimoto Makoto

  Department Of Dermatology Asahikawa Medical College

• Hashimoto Makoto

  Obihiro Univ. Agriculture And Veterinary Medicine Hokkaido Jpn

• Hashimoto Makoto

  Div. Of Applied Sci. Graduate School Of Agriculture Hokkaido Univ.

• Hashimoto Makoto

  Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin

• Hashimoto Makoto

  Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin

• Hashimoto Makoto

  Department Of Bioresource Science Obihiro University Agriculture

• Hashimoto Makoto

  Department Of Biology And Biochemistry University Of Bath:department Of Bioresource Science Obihiro

• Hashimoto Makoto

  Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin

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